Part:BBa_K3110037
Weak Promoter Strong RBS lldP+lldR+lldD
This construct has L-Lactate Permease (lldP), Regulator element (lldR) and L-Lactate Dehydrogenase (lldD) under the control of a weak promoter and strong RBS . lldP permits the entry of L-Lactate inside the bacterial cell, lldR has the dual role of an activator and a repressor and lldD cleaves L-Lactate to pyruvate . Our intention of using this part is to have control over the detection threshold of L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various higher and lower concentrations of lldP, lldR and lldD by varying the strength of the promoter and RBS controlling their production.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 328
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 3650
Illegal AgeI site found at 464
Illegal AgeI site found at 927
Illegal AgeI site found at 1365
Illegal AgeI site found at 1524
Illegal AgeI site found at 1536
Illegal AgeI site found at 2282 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3087
Characterisation
The construct could not be characterised due to insufficient time.
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